Abstract:
This work presents the development and analysis of fed-batch experiments aimed at increasing the enzyme fructosyltransferase (FTase) production. In these experiments, high cell concentration specific fructosyltransferase activity (U/g cells) were targeted. The experimental design simultaneously included the substrate/inducer addition (sucrose) not only to stimulate cell growth but also to trigger enzyme production, despite the limitations imposed by low dissolved oxygen levels. Fed-batch experiments are described, highlighting the influence of operational conditions, such as efficiency agitation speed, aeration rates, biomass production, sugar consumption and enzyme activities. The results indicate that, although the increase in oxygen transfer in Assay 2 led to greater biomass (184.8 g in 72 h), other key fermentation parameters were adversely affected, such as enzyme production, which reached a maximum value of 1440 U/g in 44 h, showing that the conditions imposed in Assay 1 were more effective despite the lower biomass with 93.6 g in 72 h, with the enzyme production reached 2230 U/g in 36 h.
Referência:
PINTO, Isabella Cristina Azarias; FERNANDES, Sérgio; RODRIGUES, Maria Filomena de Andrade; SILVA, Elda Sabino da; PERNA, Rafael Firmani; MAIORANO, Alfredo Eduardo. Effect of aeration rate and agitation speed on the fructosyltransferease production on a fed-batch process. In: CONGRESSO BRASILEIRO DE ENGENHARIA QUÍMICA, 25.; ENCONTRO BRASILEIRO SOBRE O ENSINO EM ENGENHARIA QUÍMICA, 20., 2025, Belo Horizonte. Anais… 9p.
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