Abstract:
To synthesize HER2 aptamer-conjugated iron oxide nanoparticles with a coating of poly(2-(dimethylamino) ethyl methacrylate)–poly(2-methacryloyloxyethylphosphorylcholine) block copolymer (IONPPPs). Characterization covered molecular structure, chemical composition, thermal stability, magnetic characteristics, aptamer interaction, crystalline nature and microscopic features. Subsequent investigations focused on IONPPPs for in vitro cancer cell identification. Results demonstrated high biocompatibility of the diblock copolymer with no significant toxicity up to 150 μg/ml. The facile coating process yielded the IONPP complex, featuring a 13.27 nm metal core and a 3.10 nm polymer coating. Functionalized with a HER2-targeting DNA aptamer, IONPPP enhanced recognition in HER2-amplified SKBR3 cells via magnetization separation. These findings underscore IONPPP’s potential in cancer research and clinical applications, showcasing diagnostic efficacy and HER2 protein targeting in a proof-of-concept approach.
Referência:
NEGRÃO, Cyro von Zuben de Valega; CERIZE, Natalia Neto Pereira; JUSTO JUNIOR, Amauri da Silva; LISZBINSKI, Raquel Bester; MENEGUETTI, Giovanna Pastore; ARAUJO, Larissa Ribeiro Leite de; ROCCO, Silvana A.; GONÇALVES, Kaliandra de Almeida; CORNEJO, Daniel Reinaldo; LEO, Patricia; PERECIN, Caio José(DW01); ADAMOSKI, Douglas; RODRIGUES, Sandra MarthaFIP. Gomes. HER2 aptamer-conjugated iron oxide nanoparticles with PDMAEMA-b-PMPC coating for breast cancer cell identification. Nanomedicine, v.19, n.3, 24 p., 2024.
Acesso ao artigo no site do Periódico:
