Abstract:
Fructooligosaccharides (FOS) are classified as prebiotic sugar and industrially synthesized by transfructosylation reaction in sucrose molecules catalyzed by fructosyltransferase enzymes adhered to microbial cells. The immobilization of microbial cells in support materials make it possible to obtain robust heterogeneous biocatalysts resistant to adverse conditions of the reaction medium. Loofa (Luffa cylindrica) sponges are suitable for immobilization because they have size and distribution of pores that enable the diffusion of gas and substrate, essential for microbial growth. Therefore, this work aimed to evaluate the production of cells of Aspergillus oryzae IPT-301 by submerged cell culture and their concomitant immobilization in a loofa sponge. The production occurred in a synthetic culture medium, pH 5.5, 200 rpm at 30 ºC. The microbial growth curve showed that the best cell culture time occurred after 28 h of process, obtaining a transfructosylation activity of 559.96 ± 40.37 U g-1 . The results represent the beginning of a sequence of studies about immobilization cells in loofa sponge and its biotechnological application for the enzymatic synthesis of FOS.
Referência:
TARAMELLI, Isabela A.A.; VIEIRA, Ana C.; CAVINI, Amanda P.; SANTOS, Érica D.; P. FILHO, Luís R.; FURTADO, Kalebe F.; SILVA, Elda Sabino; MORALES, Sergio A.V.; XAVIER, Michelle C.A.; PERNA, Rafael F. Immobilization of microbial cells on loofa sponge for enzymatics synthesis of fructooligosaccharides. In: CONGRESSO BRASILEIRO DE BIOTECNOLOGIA INDUSTRIAL, 1., 2024, Florianópolis. Anais… 3p.
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