Abstract:
Maintaining the enzymatic activity of microbial cells for an extended period is an industrial challenge, because prolonging the life of biocatalysts is an advantage due to reduction in process costs and consequently, reduction in environmental impact. Thus, the objective of this work was to evaluate the biochemical stability of Aspergillus oryzae IPT -301 cells immobilized in loofa sponge, preserved in a refrigerator (4 °C) in stabilizing agents for 45 days. The procedure employed involved submerged culture and simultaneous immobilization in a rotary shaker, at 30 °C, 200 rpm for 28 h. To determine enzymatic activity, the reaction was conducted in a shaking waterbath at 50 °C, 190 rpm for 60 min, then, concentrations of reducing sugar and glucose were quantified. Biocatalysts stored in Tris-Acetate buffer solution and sorbitol 70 %, pH 5.5 (4 °C) showed activities of 1479.09 ± 133.68 U g-1 and 1054.86 ± 79.16 U g-1 , respectively. It demonstrates the promising use of polyols as a stabilizing agent for stored microbial cells.
Referência:
AMARAL, Crislaine K.; VIEIRA, Ana C.; AZARIAS, Isabella; CARNEVALE, KryciaK A.; SOUZA, Bruno D.; FERNANDES, Sérgio; MAESTRELLI, Sylma C.; XAVIER, Michelle C.A.; MORALES, Sergio A.V.; PERNA Rafael F. Evaluation of biochemical stability of aspergillus oryzae IPT-301 cells immobilized in loofa sponge. In: CONGRESSO BRASILEIRO DE BIOTECNOLOGIA INDUSTRIAL, 1., 2024., Florianópolis. Anais…3p.
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