Abstract:
Fructooligosaccharides (FOS) are fructose oligomers beneficial to human health and nutrition for prebiotic sugars. Their production occurs by a transfructosylation reaction in sucrose molecules catalyzed by fructosyltransferase enzymes (FTase, E.C.2.4.1.9) adhered to microbial cells. The purpose of this work was to study the preparation, enzymatic activity, and stability of glutaraldehyde-crosslinked Aspergillus oryzae IPT301 cells used as a biocatalyst for the transfructosylation reaction of sucrose in a packed bed reactor (PBR), aiming at FOS production. The highest transfructosylation activity (AT) was presented by the biocatalyst prepared by cross-linking at 200 rpm and 45 min. The highest AT in the PBR was obtained at 50 °C, with flow rates from 3 mL min -1 to 5 mL min -1 and sucrose concentrations of 473 g L -1 and 500 g L -1 . The enzymatic kinetics was described using the Michaelis-Menten model. Finally, the biocatalyst showed constant AT of approximately 75 U g -1 and 300 U g -1 for 12 h of reaction in the PBR operating in continuous and discontinuous flow, respectively. These results demonstrate a high potential of glutaraldehydecrosslinked A. oryzae IPT-301 cells as heterogeneous biocatalysts for the continuous production of FOS in PBR reactors.
Referência:
RIBEIRO, Beatriz Menossi; DE RIN, Leandro da; JUNIOR, Sandre; DIAS, Giancarlo de Souza; XAVIER, Michelle da Cunha Abreu; SILVA, Elda Sabino da; MAIORANO, Alfredo Eduardo; PERNA, Rafael Firmani; MORALES, Sergio Andres Villalba. Cross-linked whole cerlls for the sucrose transfructosylation reaction in a continuous reactor. Chemical Industry & Chemical Engnineering Quarterly, v.30, n.2, 99-110, 2024.
Acesso ao artigo no site do Periódico:
https://www.ache-pub.org.rs/index.php/CICEQ/article/view/873
