Abstract:
Polishing entire stem and root samples is an effective method for studying their anatomy; however, polishing fresh samples to preserve woods with soft tissues or barks is challenging given that soft tissues shrink when dried. We propose sanding fresh or liquidpreserved samples under water as an alternative, given that it preserves all tissues in an intact and clear state. By manually grinding the surface of the samples under water using three ascending grits of waterproof sandpapers, an excellent polished sanded surface is obtained. The wood swarf goes into the water without clogging the cell lumina, rendering the surfaces adequate for cell visualization and description. We show results in palms, liana stems, roots, and wood blocks. Using this simple, inexpensive, rapid technique, it is possible to polish either fresh, dry, or liquid- preserved woody plant samples, preserving the integrity of both the soft and hard tissues and allowing for detailed observations of the stems and roots.
Referência:
BARBOSA, Antonio Carlos Franco; GEROLAMO, Caian S.; LIMA, André C.; ANGYALOSSY, Veronica; PACE, Marcelo R. Polishing entire stems and roots using sandpaper under water: an alternative method for macroscopic analyses. Journal Applications in Plant Sciences, v.9, n.5, May, 2021.
Access to the article on the Journal’s website:
https://bsapubs.onlinelibrary.wiley.com/doi/epdf/10.1002/aps3.11421
Polishing entire stem and root samples is an effective method for studying their anatomy; however, polishing fresh samples to preserve woods with soft tissues or barks is challenging given that soft tissues shrink when dried. We propose sanding fresh or liquidpreserved samples under water as an alternative, given that it preserves all tissues in an intact and clear state. By manually grinding the surface of the samples under water using three ascending grits of waterproof sandpapers, an excellent polished sanded surface is obtained. The wood swarf goes into the water without clogging the cell lumina, rendering the surfaces adequate for cell visualization and description. We show results in palms, liana stems, roots, and wood blocks. Using this simple, inexpensive, rapid technique, it is possible to polish either fresh, dry, or liquid- preserved woody plant samples, preserving the integrity of both the soft and hard tissues and allowing for detailed observations of the stems and roots.
Referência:
BARBOSA, Antonio Carlos Franco; GEROLAMO, Caian S.; LIMA, André C.; ANGYALOSSY, Veronica; PACE, Marcelo R. Polishing entire stems and roots using sandpaper under water: an alternative method for macroscopic analyses. Journal Applications in Plant Sciences, v.9, n.5, May, 2021.
Access to the article on the Journal’s website:
https://bsapubs.onlinelibrary.wiley.com/doi/epdf/10.1002/aps3.11421
