Abstract:
Fructooligosaccharides (FOS) production is conducted in batch bioreactors, a slow and costly process. Therefore, it is necessary to implement continuous reaction systems in fixed bed reactors (PBR) that increase the volume of FOS production and decrease its operating costs. This work evaluates the transfructosylation activity of Aspergillus oryzae IPT-301 cells immobilized in loofa sponge as a biocatalytic bed of a PBR reactor and your potential in the continuous production of FOS. The immobilization of A. oryzae IPT-301 cells in loofa sponges occurred by submerged culture using synthetic culture medium, pH 5.5, 30 ºC and 200 rpm for 28h. The reactor was operated for 180min at 50 ºC, concentration of 400 g/L of substrate and pH 5.75, with 40 loofa sponge cubes containing 0.874 ± 0.035 g of immobilized microbial cells. The transfructosylation activity was stable during the time of reaction, reaching 156.09 ± 1.38 U g-1 at steady state. The experimental results indicated the potential of the biocatalytic bed for operation in a continuous FOS production system, as the enzymatic activity profile throughout the process remained stable.
Referência:
TARAMELLI, Isabela A.A.; AMARAL, Crislaine K.; VIEIRA, Ana C.; LEITE, Paula C.; TEIXEIRA, Maria L.A.N.; SILVÉRIO, Sara I.C.; MAIORNO, Alfredo E.; MORALES, A.V.; XAVIER, Michelle C.A.; PERNA, Rafael F. Continuous system to fructooligosaccharides production with loofa sponge as na immobilization matrix. In: CONGRESSO BRASILEIRO DE BIOTECNOLOGIA INDUSTRIAL, 1., 2024, Florianópolis. Anais… 3p.
Documento com acesso restrito. Logar na BiblioInfo, Biblioteca GITEB/IPT para acessar o trabalho em PDF:
