Abstract:
This work presents the evaluation of plasmid in E. coli BL21 (DE3) along high cell density cultures in bioreactors. Two distinct constructions of plasmids were: plasmid pJ404, with the Native protein gene and the plasmid pJ411, with the Mutated protein gene. The retention of plasmid, even in high cell concentration, until 50 g/L was quite satisfactory, was around 90-100% of both plasmids. Despite results are preliminary, they indicate that, at least for the both plasmid evaluated, even in high cell density cultures, E. coli BL21 (DE3) keeps plasmids to procedure the recombinant proteins.
Reference:
RINALDI, Bruna G.; SANTOS, Murilo Marconi dos ; MELLO, Caroline Matos de ; MARTELETO, Natalia Bonifácio ; MARQUES, Telma O. ; LINHARES, Debora do Carmo ; PICCOLI, Rosane Aparecida Moniz ; LÉO, Patrícia ; RODRIGUES, Maria Filomena de Andrade . Evaluation of plasmid stability in E. coli cultivation in high cell density. In: SIMPÓSIO NACIONAL DE BIOPROCESSOS, 21., SIMPÓSIO DE HIDRÓLISE ENZIMÁTICA DE BIOMASSA, 12., 2017, Aracaju. Anais… 4 p.
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This work presents the evaluation of plasmid in E. coli BL21 (DE3) along high cell density cultures in bioreactors. Two distinct constructions of plasmids were: plasmid pJ404, with the Native protein gene and the plasmid pJ411, with the Mutated protein gene. The retention of plasmid, even in high cell concentration, until 50 g/L was quite satisfactory, was around 90-100% of both plasmids. Despite results are preliminary, they indicate that, at least for the both plasmid evaluated, even in high cell density cultures, E. coli BL21 (DE3) keeps plasmids to procedure the recombinant proteins.
Reference:
RINALDI, Bruna G.; SANTOS, Murilo Marconi dos ; MELLO, Caroline Matos de ; MARTELETO, Natalia Bonifácio ; MARQUES, Telma O. ; LINHARES, Debora do Carmo ; PICCOLI, Rosane Aparecida Moniz ; LÉO, Patrícia ; RODRIGUES, Maria Filomena de Andrade . Evaluation of plasmid stability in E. coli cultivation in high cell density. In: SIMPÓSIO NACIONAL DE BIOPROCESSOS, 21., SIMPÓSIO DE HIDRÓLISE ENZIMÁTICA DE BIOMASSA, 12., 2017, Aracaju. Anais… 4 p.
Log into BiblioInfo/IPT-DAIT to access the PDF text. Document is password protected, ask Customer Service/Library-DAIT/IPT:
https://escriba.ipt.br/pdf_restrito/174890.pdf
