Abstract:
Sugarcane juice and coffee residue (mucilage, parchment and silverskin) were investigated as complex growth media for fructosyltransferase (FTase) production by Aspergillus oryzae IPT-301. The objective of this preliminary study was the identification of low cost raw materials that lead to high enzymatic activity, thus decreasing the operational costs of an industrial process. A Plackett- Burman design was applied to determine the effects of sugarcane juice and coffee residue in the medium and the necessity of salt supplementation. The maximum transfructosylation activity obtained with the design was 12.78 U.mL-1, being the concentration of NaNO3 and the fermentation time statistically significant for the enzyme. The maximum activity ratio (At/Ah) was 23.46, being affected by the fermentation time, MnCl and coffee residue concentrations.
Reference:
MARTINS, Thais Miranda; OLIVEIRA, Marcela Fernandes de; PERNA, Rafael Firmani; SILVA, Elda Sabino da; MAIORANO, Alfredo Eduardo; BASSO, Rodrigo Corrêa. Preliminary studies of complex growth media for extracelular fructosyltransferase production from aspergillus oryzae IPT-301. In: SIMPÓSIO NACIONAL DE BIOPROCESSOS, 21., 2017, Aracaju. Anais…
Document with restricted access. To access the document in PDF, log in to BiblioInfo in the Library/DAIT-IPT:
https://escriba.ipt.br/pdf_restrito/174790.pdf
Sugarcane juice and coffee residue (mucilage, parchment and silverskin) were investigated as complex growth media for fructosyltransferase (FTase) production by Aspergillus oryzae IPT-301. The objective of this preliminary study was the identification of low cost raw materials that lead to high enzymatic activity, thus decreasing the operational costs of an industrial process. A Plackett- Burman design was applied to determine the effects of sugarcane juice and coffee residue in the medium and the necessity of salt supplementation. The maximum transfructosylation activity obtained with the design was 12.78 U.mL-1, being the concentration of NaNO3 and the fermentation time statistically significant for the enzyme. The maximum activity ratio (At/Ah) was 23.46, being affected by the fermentation time, MnCl and coffee residue concentrations.
Reference:
MARTINS, Thais Miranda; OLIVEIRA, Marcela Fernandes de; PERNA, Rafael Firmani; SILVA, Elda Sabino da; MAIORANO, Alfredo Eduardo; BASSO, Rodrigo Corrêa. Preliminary studies of complex growth media for extracelular fructosyltransferase production from aspergillus oryzae IPT-301. In: SIMPÓSIO NACIONAL DE BIOPROCESSOS, 21., 2017, Aracaju. Anais…
Document with restricted access. To access the document in PDF, log in to BiblioInfo in the Library/DAIT-IPT:
https://escriba.ipt.br/pdf_restrito/174790.pdf
