Superparamagnetic nanoparticles synthesized with poloxamer 188 for biological applications

Abstract:

Superparamagnetic nanoparticles encapsulated or functionalized with polymers are a promising theranostic agent, as they can perform therapeutic and diagnostic functions. In this work, iron oxide nanoparticles were synthesized by coprecipitation with (Mag-P) and without (Mag) the presence of Poloxamer 188, with proposal to functionalize the particles. X-Ray Diffraction (XRD), Infrared Spectroscopy (FT-IR), Dynamic Light Scattering (DLS), magnetization versus temperature and applied field H, Thermogravimetric Analysis (TGA) and cytotoxicity for normal and tumor cells were performed to characterize the particles obtained. XRD and FT-IR analysis confirmed the formation of magnetite in both samples and the presence of Poloxamer in Mag-P, determined as 3 % by TGA. The hydrodynamic diameters measured by DLS were of 53 nm for Mag and 180 nm for Mag-P, but magnetization versus temperature curves showed that they are superparamagnetic at room temperature, which indicates that they are composed of magnetite nanoparticles smaller than that. Mag’s blocking temperature is 210 K and Mag-P’s is 172 K. Saturation moment at 300 K is of 72.1 emu/g for Mag and 66.6 emu/g for Mag-P. Cytotoxicity evaluation indicated that the particles are not toxic to the tumor cells HeLa and HepG2 and are very safe for the healthy cells NCTC 929. It is expected that the magnetite nanoparticles with Poloxamer can be tested for biological applications, as to treat tumors by magnetic hyperthermia or to enhance MRI contrast for diagnostics.


Reference:
PERECIN, Caio J.; YOHIOKA, Sergio A.; CHITTA, Valmir A.; GRATENS, Xavier P.M.; LÉO, Patrícia; OLIVEIRA, Adriano Marim de; CERIZE, Natália Neto Pereira . Superparamagnetic nanoparticles synthesized with poloxamer 188 for biological applications. In: SMM 22, SOFT MAGNETIC MATERIALS CONFERENCE, 2015., São Paulo. Proceedings… 4 p.

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