Abstract:
Biocorrosion or Microbial Induced Corrosion (MIC) is a process caused by anaerobic microorganisms, especially sulfate-reducing bacteria (SRB). Checking the presence and concentration of these microorganisms is part of the preventive measures routine against corrosion in several stages of oil and gas exploitation. In this article, the development process of an impedimetric biosensor to rapidly detect SRB using specific antibody is described. The target is the APS-reductase enzyme, which is involved in the biocorrosion process and presents common characteristics into the SRB group, allowing the indirect detection and quantification of SRB. Specific monoclonal and polyclonal antibodies were successfully obtained after animal immunization using a synthetic peptide as antigen, which is representative of APS-reductase enzyme. Both monoclonal and polyclonal antibodies (anti APS-reductase) proved to be SRB specific by Dot Immuno Binding Assay (DIBA). Monoclonal antibodies anti APS-reductase was immobilized in commercial Screen-Printed Electrodes (SPE), which were first modified at the surface by a self-assembled monolayer (SAM) technology using cysteamine coupling. Electrochemical Impedance Spectroscopy (EIS) was selected as the analytical tool for evaluating the biosensor performance. Results have shown that this type of sensor is suitable to detect different antigen (APS-reductase synthetic peptide) concentration. At this point, anti APS-reductase biosensors are being prepared to be tested with SRB lysate from pure culture and from SRB field sample. Main challenges are related to ensure repeatability of results using commercially available SPEs, and also to overcome possible interferences present in field samples.
Reference
LÉO, Patrícia; LINHARES, Débora do Carmo; RODRIGUES, Maria Filomena de A.; GUIMARÃES, Kleber Lanigra; GONGORA-RUBIO, Mário Ricardo; GOMES, Jonas de Carvalho; MONTEMOR, Antônio Fernando; OLIVEIRA, Douglas H.S.; SILVA, Beatriz A.A.; RAMOS, Luciana Wasnievski da Silva de Luca. Rapid detection of corrosion inducing microorganisms using antibodies as bioreceptors for its application into microdevices. In: RIO OIL AND GAS EXPO AND CONFERENCE, 2016, Rio de Janeiro. Proceedings… 7 p.
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https://escriba.ipt.br/pdf_restrito/174355.pdf
