Abstract:
Fructooligosaccharides (FOS) are fructose oligomers whose fructosyl units are attached at the β (2-> 1) position in the sucrose molecule. These sugars are classified as prebiotics and present several benefits to human health. FOS are commercially available through synthetic production using microbial enzymes such as fructosyltransferase (FTase, E.C.2.4.1.9) and sucrose with substrate. The aim of this work was to study the extracellular FTase production from of Aspergillus oryzae IPT-301 by submerged fermentation using synthetic culture medium, and to perform the biochemical characterization of the enzyme through the evaluation of the effects of pH (3.0 ≤ pH ≤ 6.5) and the temperature (30 ° C ≤T≤65 ° C) of the reaction medium in the hydrolytic (Ah) and transfructosylation (At) activities. FTases were produced with the highest transfructosylation activities (19.76 + – 0.56 U.mL-1) and lower hydrolytic activities (1.65 + – 0.31 U.mL-1), rat 64 h of fermentation, resulting in a ratio between activities (At / Ah) equal to 12.22 + – 1.82. Regarding the biochemical characterization, the enzyme presented maximum transfructosylation activity and minimal hydrolytic activity in the reaction medium for a pH range of 4.5-6.0 and a temperature of 50ºC. The results suggest that the fungus is a promising source for production of extracellular FTases for the synthesis of FOS in laboratory scale.
Reference:
PERNA, Rafael Firmani; CUNHA, Josivan de Sousa; MORALES, Sergio Andres Villalba; XAVIER, Michelle da Cunha Abreu; OTTONI, Cristiani Angelica; SILVA, Elda Sabino; MAIORANO, Alfredo Eduardo. Produção e caracterização bioquímica de frutosiltransferase extracelular microbiana para a síntese de frutiikigossacarídeos em escala laboratorial. In: SANTOS, Alberdam Silva. Avanços científicos e tecnológicos em bioprocessos. Ponta Grossa : Athena Ed., 2018. Cap. 2, p.7-22.
Fructooligosaccharides (FOS) are fructose oligomers whose fructosyl units are attached at the β (2-> 1) position in the sucrose molecule. These sugars are classified as prebiotics and present several benefits to human health. FOS are commercially available through synthetic production using microbial enzymes such as fructosyltransferase (FTase, E.C.2.4.1.9) and sucrose with substrate. The aim of this work was to study the extracellular FTase production from of Aspergillus oryzae IPT-301 by submerged fermentation using synthetic culture medium, and to perform the biochemical characterization of the enzyme through the evaluation of the effects of pH (3.0 ≤ pH ≤ 6.5) and the temperature (30 ° C ≤T≤65 ° C) of the reaction medium in the hydrolytic (Ah) and transfructosylation (At) activities. FTases were produced with the highest transfructosylation activities (19.76 + – 0.56 U.mL-1) and lower hydrolytic activities (1.65 + – 0.31 U.mL-1), rat 64 h of fermentation, resulting in a ratio between activities (At / Ah) equal to 12.22 + – 1.82. Regarding the biochemical characterization, the enzyme presented maximum transfructosylation activity and minimal hydrolytic activity in the reaction medium for a pH range of 4.5-6.0 and a temperature of 50ºC. The results suggest that the fungus is a promising source for production of extracellular FTases for the synthesis of FOS in laboratory scale.
Reference:
PERNA, Rafael Firmani; CUNHA, Josivan de Sousa; MORALES, Sergio Andres Villalba; XAVIER, Michelle da Cunha Abreu; OTTONI, Cristiani Angelica; SILVA, Elda Sabino; MAIORANO, Alfredo Eduardo. Produção e caracterização bioquímica de frutosiltransferase extracelular microbiana para a síntese de frutiikigossacarídeos em escala laboratorial. In: SANTOS, Alberdam Silva. Avanços científicos e tecnológicos em bioprocessos. Ponta Grossa : Athena Ed., 2018. Cap. 2, p.7-22.
