Abstract:
Small -angle scattering (SAS) is a powerful tool to investigate structural properties of biomolecular systems in solution. In particular, size, shape and conformational states of the toxins/proteins can be determined along with their interactions depending on the environment. In the current chapter, an overview of the basic theory and data analysis including determination of protein radius of gyration through Guinier’s law and distance distribution function p(r) will be presented and discussed. Particular emphasis will be placed to examples containing proteins in folded and unfolded states and aggregated states.
Reference:
ITRI, Rosangela; SALES, Elisa Morandé; BARBOSA, Leandro R.S. Espalhamento de raios-X a baixos ângulos (SAXS) aplicado ao estudo de proteínas não interagentes em solução. In: SANTOS, Fabiola Pazos; VALCÁRCEL, Carlos Álvarez. Estrategias y avances em el estúdio de toxinas de interés para la biomedicina. Puebla: Benemétria Universidad Autonoma de Pueblam, 2015. Cap.7, p.171-190.
Access to the book in its entirety on the Library-DAIT/IPT website:
Small -angle scattering (SAS) is a powerful tool to investigate structural properties of biomolecular systems in solution. In particular, size, shape and conformational states of the toxins/proteins can be determined along with their interactions depending on the environment. In the current chapter, an overview of the basic theory and data analysis including determination of protein radius of gyration through Guinier’s law and distance distribution function p(r) will be presented and discussed. Particular emphasis will be placed to examples containing proteins in folded and unfolded states and aggregated states.
Reference:
ITRI, Rosangela; SALES, Elisa Morandé; BARBOSA, Leandro R.S. Espalhamento de raios-X a baixos ângulos (SAXS) aplicado ao estudo de proteínas não interagentes em solução. In: SANTOS, Fabiola Pazos; VALCÁRCEL, Carlos Álvarez. Estrategias y avances em el estúdio de toxinas de interés para la biomedicina. Puebla: Benemétria Universidad Autonoma de Pueblam, 2015. Cap.7, p.171-190.
Access to the book in its entirety on the Library-DAIT/IPT website:
